A Proteomic Analysis of Human Uterine Myoma

Author(s): Antonio Rizzello, Julien Franck, Marcello Pellegrino, Francesco De Nuccio, Pasquale Simeone, Giovanni Fiore, Silvia Di Tommaso, Antonio Malvasi, Andrea Tinelli, Isabelle Fournier, Michel Salzet, Michele Maffia, Daniele Vergara

Journal Name: Current Protein & Peptide Science

Volume 18 , Issue 2 , 2017


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Abstract:

Uterine leiomyoma is a benign smooth muscle tumor characterized by a high incidence in women of reproductive age. The aetiology of this tumor is still unknown but established risk factors include high levels of female hormones, family history, African ancestry, early age of menarche and obesity. Here, to identify proteomic features associated with this tumor type, we performed a liquid chromatography-mass spectrometry (LC-MS/MS) analysis of uterine myomas. The identified proteins were subjected to a gene ontology analysis to generate biological functions, molecular processes, and protein networks that were relevant to the uploaded dataset. Pathway-based analysis was an effective approach to investigate the molecular mechanisms underlying the disease and to create biological hypotheses about regulation of our proteins including the identification of upstream regulators and main protein nodes. Moreover, proteomic and in silico data were combined with immunohistochemistry and western blotting to identify a group of proteins representative of some selected pathways, with a dysregulated expression in myoma, pseudocapsule, and normal myometrium samples. Based on these results, we confirmed the over-expression of extracellular matrix components, and estrogen and progesterone receptors in uterine myomas, and proposed biological networks, canonical pathways and functions that may be relevant to the pathophysiology of this tumor.

Keywords: Uterine fibroids, mass spectrometry, system biology, pseudocapsule, estrogen receptor, progesterone receptor, extracellular matrix.

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Article Details

VOLUME: 18
ISSUE: 2
Year: 2017
Page: [167 - 174]
Pages: 8
DOI: 10.2174/1389203717666160322150603
Price: $65

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