Title:Anti-Aging Property of G2013 Molecule as a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model
VOLUME: 13 ISSUE: 1
Author(s):Abbas Mirshafiey, Soma Hosseini, Sanaz Afraei, Noshin Rastkari, Farzaneh T. Zavareh and Gholamreza Azizi
Affiliation:Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Keywords:CAT, cortisol, G2013, GPX1, GST, iNOS2, MDA, MPO, oxidative stress, PCO, ROS, SOD2,
TAC.
Abstract:Background: G2013 molecule is a novel non-steroidal anti-inflammatory agent with
immunosuppressive property, which was investigated on determinants relative to the oxidative stress
in animal model. Materials and Methods: The Sprague-Dawley rats were used for evaluating
properties of G2013 on some oxidative stress enzymes including: Myeloperoxidase (MPO),
Glutathione peroxidase (GPX1), mitochondrial Superoxide dismutase (SOD2), Catalase (CAT),
Glutathione S-Transferase (GST), and inducible nitric oxide synthase (iNOS) genes expression by
Real Time PCR. The rats were sacrificed 3 months after daily oral administration of G2013.
Moreover, Malondialdehyde (MDA), Carbonyl protein (PCO), the lipid and protein oxidation markers
respectively and total anti-oxidant capacity (TAC) were tested in serum by biochemical analysis. Also
cortisol as a steroid hormone was evaluated by chemiluminescence immunoassay after 12 weeks
consumption of G2013 solution. Result: Our findings revealed a significant decrease in MPO in
G2013 treated group, indicating its favorable effects but has no significant effects on genes expression
of another antioxidant enzymes, including: SOD2, CAT, GPX1, and GST. Also, there were no
significant differences in PCO, TAC and cortisol compared to control group following G2013
consumption. While an enhancement in serum MDA level was observed in the treatment group. In
addition, G2013 therapy did not show any weight loss. Conclusions: Our data showed the safety and
efficacy of G2013 as a novel designed NSAID on various oxidative stress determinants.
