Background: Marrubium vulgare is used in traditional medicine in some countries such as
Jordan in the treatment of diabetes and wounds. The plant is reported to possess vasorelaxant, antihypertensive,
analgesic, anti-inflammatory and antioxidant properties. Earlier phytochemical investigations
of M. vulgare led to the characterization of the diterpene marrubiin (2) which is the major constituent
of the plant , and exhibits potent antinociceptive properties and vasorelaxant activity, marruboside
and marrubic acid. The aim of this work is to isolate and identify new compounds from Jordanian
Method: The crude ethanolic extract of air- dried defatted whole plants was partitioned into aqueous methanol (MA), butanol
(MB) and hexane extracts. The aqueous methanol and butanol extracts were chromatographied on a silica gel columns.
Each fraction was purified by a combination of CC, TLC and/ or recrystalization using suitable solvent systems to
produce 10 compounds. NMR, MS, and UV spectral analyses were used for structure elucidation of the isolated compounds.
Results: The methanol extract yielded eight pure compounds, which were identified as the new compound apigenin7-O-
(3",6"- E-p-dicoumaroyl)-β-glucoside 1 and the known compounds marrubiin 2, the major constituent of Marrubuim vulgare,
pectolinarigenin 3, apigenin-7-methyl ether 4, labd-13(14)-en-9a, 15, 16-triol 5, apigenin 6, β-sitosteryl glucoside 7,
apigenin-7-O-(6"-O- E-p-coumaroyl)-β-glucoside 8. In a similar manner, the butanol extract (MB) was treated as mentioned
with the (MA) extract and the resulting fraction afforded the two known compounds: luteolin-7-O-glucoside 9 and
acteoside 10 which is reported for first time from Marrubium vulgare.
Conclusion: The treatment of the air- dried defatted whole plant of Marrubium vulgare led to the isolation of ten pure
compounds, two of them were isolated for the first time from this plant: apigenin7-O-(3",6"- E-p-dicoumaroyl)-β-
glucoside 1and acteoside 10.