Novel Methods of Genetic Modification of Human Pluripotent Stem Cells

Author(s): Maria V. Camarasa

Journal Name: Recent Patents on Regenerative Medicine
Continued as Current Regenerative Medicine

Volume 5 , Issue 2 , 2015


Abstract:

Genomic engineering has enormous potential along basic research, drug discovery and cell therapeutics. Many existing methods for targeted gene knockout mutagenesis or integration rely on homologous recombination. The low rate of spontaneous recombination in nearly all mammalian cell types, as well as the scale of screening, effort and time required to isolate the targeted events during genome modification, have hindered progress in this field. The present review has the objective to present latest improvements of technology to develop genetic modification to a clinical grade level so it can be used in human therapy.

Keywords: Genomic editing, naive pluripotent stem, patient-specific, pluripotent, stem cell/genetic therapy.

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Article Details

VOLUME: 5
ISSUE: 2
Year: 2015
Published on: 07 March, 2016
Page: [125 - 144]
Pages: 20
DOI: 10.2174/2210296506666160204231756
Price: $58

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