Structure-Based Virtual Screening for Defeating Drug Resistant Form of EGFR Protein

Author(s): Amirhossein Sharifi, Kowsar Bagherzadeh, Sahand Golestanian, Massoud Amanlou

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 19 , Issue 3 , 2016

Become EABM
Become Reviewer
Call for Editor


Epidermal growth factor receptor (EGFR) is a tyrosine kinase with a key role in cell proliferation, death and differentiation. Mutations in EGFR, including substitution of Thr790 by methionine and Leu858 by arginine (T790M/L858R), lead to a lung cancer that is resistant against first generation inhibitors. In fact, second generation inhibitors were developed, but they proved to have had severe side effects because of the significant potency to suppress the wild type protein just as much. To resolve the problem, a step-by-step rational virtual screening was employed over almost sixty million compounds of PubChem Compound Database to filter out selective inhibitor(s) of T790M/L858R subtype. Consequently, the compound CID 133077 was observed, an active metabolite of Axitirome and also a cholesterol lowering prodrug. Selecting this compound can be explained by the oxamic acid part of molecule. Hence, administration of Axitirome or other compounds which contain oxamic acid is suggested in cases with EGFR T790M/L858R drug resistance.

Keywords: EGFR, rational virtual screening, docking studies, molecular dynamic simulations, pharmacophore search, Axitirome, CID 133077.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2016
Published on: 21 March, 2016
Page: [228 - 237]
Pages: 10
DOI: 10.2174/1386207319666160115132121
Price: $65

Article Metrics

PDF: 32