Introduction: A green analytical method simple, rapid and economic was validated for the
determination of cefepime hydrochloride and has been developed using reversed phase high performance
liquid chromatography (RP-HPLC) in pharmaceutical preparations.
Methods: The liquid chromatography method was carried out using as mobile phase a mixture of ethanol
and water (55:45, v/v), on column Luna C18 (250 x 4.6 mm; 5 µm) at a flow rate of 0.5 mL min-1 with
UV detection at 258 nm.
Results: The method was validated according to ICH guidelines, presenting optimum linearity in the
range 10-70 µg mL-1 (r = 1.0000) and retention time (tR) equal to 4.99 minutes. Statistical analyses were
performed for the validated method, proving the safety and reliability of the results. Other important factors
is that the method uses less amounts of organic solvent, produces low levels of waste and does not
use buffer solution, minimizing effluent treatment, which contributes to the environment and implements
methods aimed green chemistry, making economic for the industry.
Conclusion: Thus, the method can be employed as an alternative in quality control routine analysis of
pharmaceutical industry for quantification of cefepime hydrochloride lyophilized powder for solution for