Background: Asenapine is a new antipsychotic drug used in the treatment of schizophrenia
and bipolar disorder. Paroxetine and fluvoxamine are second generation antidepressant drugs.
Objective: Herein we have developed a simple and rapid UHPLC-DAD method to determine asenapine,
fluvoxamine and paroxetine. The methodology was applied to the analysis of human biofluids, namely
blood serum, urine and cerebrospinal fluid.
Method: The analytes of interest were separated on an Inertsil C8, (250 x 4.0 mm) 5 µm, analytical column,
using mobile phase consisted of acetate buffer (0.2 M, pH=4.5), methanol and acetonitrile (60:10:30
% v/v). Isocratic elution was performed at a flow rate of 1.7 mL/min. Photodiode array detection was
applied at 231 nm for asenapine, 294 nm for paroxetine and 251 nm for fluvoxamine. Sulfadimethoxine
(2.5 ng/µL) was chosen as the internal standard. The method was validated in terms of linearity, selectivity,
accuracy, precision and stability.
Results: Limits of detection and quantitation were 0.03 ng/µL, and 0.10 ng/µL respectively, using the
S/N= 3.3 and S/N= 10 criteria.
Conclusion: The developed method is consistent and can be used as a useful analytical tool to monitor
the three drugs in serum, urine and cerebrospinal fluid, in patients under treatment.