Background: Vigabatrin is a variant of the inhibitory neurotransmitter γ-aminobutyric acid
(GABA). It reduces seizure activities by inhibiting irreversibly GABA transaminase and increasing
Objective: This study presents simple, sensitive, extraction-free, and cost-efficient estimation of vigabatrin
using a spectrophotometric method in pharmaceutical formulation.
Method: The method relies on the development of water-soluble, blue-violet colored complexes of vigabatrin
and ninhydrin in a phosphate buffer of pH 7.4 with λmax at 565 nm. Linearity, accuracy, precision,
and specificity were performed as validation parameters for this method.
Results: Assessment of different analytical factors was carried out, and statistical analysis was performed
to validate the results. The findings showed a linear progression in absorbance when the concentration of
vigabatrin was increased. The correlation coefficient value (r2) of 0.9981 was observed. The operations
followed the Beer’s law in the span of 40-200 µgmL-1 with a molar absorptivity of 5.16x103 Lmol–1cm–1.
Recovery values in the marketed formulation of the assay method indicated no interference from the
common additives and excipients. The limits of detection and quantification were found to be 6.0 and 40
Conclusion: The presented method has demonstrated to be delicate, reliable, explicit, and intuitive that
can prove beneficial for regular laboratory examination of vigabatrin in pharmaceutical dosage forms.