Crocin is an apocarotenoid present in Nyctanthes arbortrisits (NAT) flower calyx extract in
abundant quantity of about 36.7% w/w. The carotenoid rich extract of NAT exhibited dose dependant
anti-angiogenic effect in chick-chorioallantoic membrane (CAM) assay. The anti-angiogenic activity
of NAT extract (1 mg/ml) was observed to be equivalent to Heparin (1 mg/ml) with the IC50 value of
0.5 mg/ml. The antiangiogenic activity of NAT was further confirmed by in-vivo Matrigel Matrix
model of Balb/c mice with VEGF inhibitor, sunitinib malate (1 μM) as the anti-angiogenic control.
The in-vivo study revealed significant (P<0.01) reduction in hemoglobin levels and inhibited angiogenesis as compared to
angiogenic control viz. VEGF loaded matrigel matrix. The anti-angiogenic activity of NAT calyx ethanolic extract is
likely to be due to the presence of Crocin since carotenoids possess inherent strong anti-oxidant potential. Our result
brings out a new naturally occurring anti-angiogenic agent which may find its utility in the treatment of conditions like
cancer, diabetic retinopathy and cataract.
Keywords: Nyctanthes arbourtristis, carotenoids, CAM, anti-angiogenic, matrigel matrix, crocin.
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