Recent Advances in Medicinal Chemistry

Volume: 2

Indexed in: EBSCO.

Recent Advances in Medicinal Chemistry is a book series focused on leading-edge research on developments in rational drug design, synthetic chemistry, bioorganic chemistry, high-throughput screening, ...
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Analysis and Occurrence of Flavonoids in Foods and Biological Samples

Pp. 10-58 (49)

Celestino Santos-Buelga, Ana M. González-Paramás, Susana González- Manzano and Montserrat Dueñas


Flavonoids are a major class of plant phenolics that are widely distributed in the human diet and have been related to health promotion. They may occur in their natural sources in free forms (aglycones), as glycosylated or acylated derivatives, or as oligomeric and polymerized structures. This structural diversity affects their physicochemical behaviour and complicates their analysis. Thus, there is not a single standardized procedure that can be recommended for all flavonoid groups and/or type of samples, and the procedures have to be optimized depending on the nature of the sample and the target analytes. Furthermore, when dealing with the analysis of flavonoids biological samples (i.e., human and animal fluids and tissues) some differential aspects have to be taken into account; the nature of the compounds that can be found in those samples may differ from that present in plants and food, and flavonoids and metabolites occur in much lower concentrations, which make their analysis still more challenging. In this review the main techniques for extraction and analysis of flavonoids in foodstuffs and biological fluids are revised. Discussion on available databases including data on the contents of flavonoids in foods, useful for the estimation of their dietary intake, and emerging biomarkers of flavonoid consumption is also made.


Analysis, biological fluids, biomarkers, extraction, flavonoid metabolites, flavonoids, foods, HPLC, identification, LC-MS, occurrence in food, phytochemical databases, polyphenols, sample preparation.


Grupo de Investigación en Polifenoles (GIP/USAL), Universidad de Salamanca, Unidad de Nutrición y Bromatología, Facultad de Farmacia, Campus Miguel de Unamuno, E-37007. Salamanca, Spain.