Title:Identification of Differentially Expressed Proteins During Regeneration of Dendrorhynchus zhejiangensis Using the Comparative Proteomic Analysis
VOLUME: 12 ISSUE: 3
Author(s):Ye Li, Lei Chen, Jun Zhou, Xiurong Su and Chundan Zhang
Affiliation:School of Marine Sciences, Ningbo University, Ningbo, 315211 China.
Keywords:Two-dimensional gel electrophoresis, gelsolin-like protein, actin; nemertean, regeneration, expression.
Abstract:In order to examine underlying mechanisms of the impressive regeneration in Dendrorhynchus
Zhejiangensis, a two-dimensional gel electrophoresis (2-DE) combined with mass spectrometric
analysis was employed. 70 proteins involved in regenerative process were identified and 33 proteins
were up-regulated and others were down-regulated in fracture injured model. Among them, about 79%
were cell cytoskeleton proteins and motor proteins. As the representatives, the expression profiles of
Actin and Gelsolin-like protein (GSN), both of which were involved in cell motility, in response to
body breakage of nemerteans were examined. Two genes showed similar expression patterns during
regeneration process and both of them were first constantly up-regulated at 1h and 3h after being broken,
then down-regulated to a lower level at 3rd day. The results suggested that Actin and GSN play important roles in regeneration
of D. Zhejiangensis.
