New hollow fiber based liquid phase microextraction (HF-LPME) technique
followed by high performance liquid chromatography (HPLC) coupled with ultraviolet
(UV) detector was introduced for detection and determination of terazosin in urine and
plasma samples. The effect of different parameters which influence extraction
efficiency such as organic membrane solvent, donor/acceptor phase composition, extraction
time, stirring rate, salt concentration and extraction temperature were studied.
Following optimization, extraction recoveries more than 86% were obtained in different
biological matrices which resulted in preconcentration factors higher than 161 and
an acceptable repeatability (1.25 < RSD% <2.30, n=3). The method presents good
linearity with coefficiency higher than 0.9994. Finally, the proposed method was applied
for detection and determination of terazosin in urine and plasma samples.