Title:Green Propolis: Thirteen Constituents of Polar Extract and Total Flavonoids Evaluated During Six Years through RP-HPLC
VOLUME: 12 ISSUE: 4
Author(s):Sonia Maria de Figueiredo, Nancy Scardua Binda, Bruno de Moura Almeida, Sheila Rago Lemos Abreu, José Alexandre Silva de Abreu, Gláucia Maria Pastore, Hélia Harumi Sato, Viviane Cristina Toreti, Eulália Vargas Tapia, Yong Kun Park, Sidney Augusto Vieira Filho and Rachel Basques Caligiorne
Affiliation:Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte (IEP/SCBH), Rua Domingos Vieira, 590, Belo Horizonte, 30150-240, Minas Gerais, Brazil.
Keywords:Artepillin C, Baccharis dracunculifolia, Flavonoids, Green Propolis, p-Coumaric Acid, RP-HPLC.
Abstract:In order to verify the chemical qualities of polar extract of Green Propolis produced in the
State of Minas Gerais, Brazil, was analyzed by means of RP-HPLC, the concentration of eleven
flavonoids, Artepillin C and p-coumaric acid were analyzed by means of RP-HPLC. Samples were
collected in the months of February, March, May, July, September, and October, during a period of six
years (2008 to 2013) and the results compared with purchased standards. Artepillin C was the main
constituent (80-90%) found in all samples. Lower concentrations of p-coumaric acid, chrysin and
kaempferide were observed in March, and of ricin and galangin in September. Respectively, these
months correspond to the end of Summer and Spring period in Brazil. The variation in concentration of
the analyzed constituents always occurred in the same month, during the entire six years of evaluation
period. All thirteen constituents of Green Propolis were detected throughout the study period, and the
average concentration of each one was similar in relation to the respective monthly collection period of
each year. Due to similarities among chemical constituents of Green Propolis with those present in B.
dracuncufolia, this plant was identified as being the principal source of Green Propolis.
