In order to verify the chemical qualities of polar extract of Green Propolis produced in the
State of Minas Gerais, Brazil, was analyzed by means of RP-HPLC, the concentration of eleven
flavonoids, Artepillin C and p-coumaric acid were analyzed by means of RP-HPLC. Samples were
collected in the months of February, March, May, July, September, and October, during a period of six
years (2008 to 2013) and the results compared with purchased standards. Artepillin C was the main
constituent (80-90%) found in all samples. Lower concentrations of p-coumaric acid, chrysin and
kaempferide were observed in March, and of ricin and galangin in September. Respectively, these
months correspond to the end of Summer and Spring period in Brazil. The variation in concentration of
the analyzed constituents always occurred in the same month, during the entire six years of evaluation
period. All thirteen constituents of Green Propolis were detected throughout the study period, and the
average concentration of each one was similar in relation to the respective monthly collection period of
each year. Due to similarities among chemical constituents of Green Propolis with those present in B.
dracuncufolia, this plant was identified as being the principal source of Green Propolis.