Title:Applications of luminous bacteria enzymes in toxicology
VOLUME: 18 ISSUE: 10
Author(s):Valentina A. Kratasyuk and Elena N. Esimbekova
Affiliation:Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russia.
Keywords:Bioluminescence, bioluminescent toxicity enzymatic assay, immobilization of enzymes, luciferase, total toxicity.
Abstract:This review describes the principle and applications of bioluminescent enzymatic toxicity
bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase
and luciferase to replace living organisms in developing cost-competitive biosensors for
environmental, medical and industrial applications. These biosensors instantly signal chemical and
biological hazards and allow for detecting a great amount of toxic compounds with advantages
associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
