Applications of luminous bacteria enzymes in toxicology

Author(s): Valentina A. Kratasyuk, Elena N. Esimbekova

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 18 , Issue 10 , 2015


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Abstract:

This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.

Keywords: Bioluminescence, bioluminescent toxicity enzymatic assay, immobilization of enzymes, luciferase, total toxicity.

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Article Details

VOLUME: 18
ISSUE: 10
Year: 2015
Published on: 03 November, 2015
Page: [952 - 959]
Pages: 8
DOI: 10.2174/1386207318666150917100257
Price: $65

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