Creation of artificial luciferases to expand their analytical potential

Author(s): Ludmila A. Frank

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 18 , Issue 10 , 2015


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Abstract:

Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescencebased analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc.

Keywords: Luciferase, luciferin, photoprotein, bioluminescence, mutagenesis, luciferase-based assay, bioimaging, reporter assay.

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Article Details

VOLUME: 18
ISSUE: 10
Year: 2015
Published on: 03 November, 2015
Page: [919 - 929]
Pages: 11
DOI: 10.2174/1386207318666150917100011
Price: $65

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