Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro

Title:Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro

Volume: 22 Issue: 11

Author(s): Kyu-Yeon Han, Michael Chang, Hong-Yu Ying, Hyun Lee, Yu-hui Huang, Jin-Hong Chang and Dimitri T. Azar

Affiliation:

Keywords: Collagen XVIII, endostatin, neostatin, VEGFR1, VEGFR2 and VEGFR3.

Abstract: We previously reported that neostatin, a proteolytic fragment of collagen XVIII that includes endostatin, inhibits basic fibroblast growth factor-induced corneal angiogenesis and lymphangiogenesis. In experiments to determine which fragments in neostatin are responsible for binding to VEGF receptors (VEGFRs), we previously showed that a 28- mer sequence at the C-terminal of endostatin, known as endostatin peptide 9, preferentially binds VEGFR3-Fc over VEGFR1-Fc and VEGFR2-Fc. In the present study, we show that a different endostatin fragment, endostatin peptide 4 (26 mers long), also selectively binds VEGFR3-Fc and not VEGFR1-Fc or VEGFR2-Fc. From surface plasmon resonance data, the K_D and Chi² (RU²) values for endostatin peptide 4 binding to VEGFR3-Fc are 5.72x10^-8 M and 0.354, respectively. In conclusion, endostatin peptides 4 and 9 may be responsible for endostatin binding to VEGFR3-Fc, and this improved understanding of endostatin peptide binding to VEGFR3-Fc may support the development of therapeutics targeting lymphangiogenic processes.

Cite this article as:

Han Kyu-Yeon, Chang Michael, Ying Hong-Yu, Lee Hyun, Huang Yu-hui, Chang Jin-Hong and Azar T. Dimitri, Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro, Protein & Peptide Letters 2015; 22 (11) . https://dx.doi.org/10.2174/0929866522666150907111953