Resveratrol Protects Endothelial Cells from Rapid Stretch Injury and Hypoxia In Vitro

Author(s): Elke Sokoya

Journal Name: Current Drug Therapy

Volume 10 , Issue 1 , 2015


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Abstract:

Background: An important function of endothelial cells is to maintain a balance between the production of vasodilators such as nitric oxide (NO) and vasoconstrictors such as endothelin-1, thereby maintaining vascular tone and blood flow. However in many disease states, impaired production of NO leads to vasoconstriction, platelet aggregation and oxidative stress. Resveratrol, a polyphenolic compound found in peanuts and red wine, has been shown to induce NO production.

Objective: The purpose of the present study was to determine the effect of resveratrol on cultured brain endothelial cells in two models of pathology characterized by disrupted NO production – hypoxia alone and then a combination regimen of rapid stretch injury followed by hypoxia.

Method: Mouse brain endothelial cells cultured in resveratrol caused a dose-dependent increase in endothelial nitric oxide synthase (eNOS) protein expression. Importantly, resveratrol prevented hypoxia-induced decrease in eNOS expression. This was complemented by reduced cell death, as evidenced by a reduction in propidium iodide staining. However coincubation of resveratrol with the NO inhibitor, L-NAME, did not prevent this protection. Rapid stretch injury and hypoxia produced marked cell necrosis and resveratrol significantly inhibited this death.

Results: Our results suggest that resveratrol can prevent hypoxia-induced blunting of eNOS expression and protects endothelial cells from hypoxia alone and a combined regimen of rapid stretch injury and hypoxia.

Conclusion: This study has shown for the first time that resveratrol prevents hypoxia-induced decrease in eNOS expression and protects endothelial cells from hypoxia alone and a combined regimen of rapid stretch injury and hypoxia.

Keywords: Endothelium, nitric oxide, resveratol.

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Article Details

VOLUME: 10
ISSUE: 1
Year: 2015
Published on: 24 August, 2015
Page: [56 - 64]
Pages: 9
DOI: 10.2174/157488551001150825100709

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