Background: L-Asparaginase (LA) has wide applications due to anticancer activity.
However, limitations involved with commercially available LA (e.g. causing hypersensitivity)
call for searching new sources of LA with less adverse effects. Marine-derived fungi
represent a potential source for LA that has not been fully explored. Objective: To screen
marine-derived fungi –untapped source for L-Asparaginase production. Methods: Marinederived
fungi isolated from Bhavnagar coast, Gulf of Khambhat, West coast of India, were
screened for L-Asparaginase production by agar plate assay and broth assay. Quantification of L-asparaginase
activity was carried out by estimating liberation of ammonia. Results: Fourteen marine-derived fungi out of
twenty isolates tested showed L-Asparaginase production as revealed by development of pink zone around
colony on agar plates and development of pink color in flask with increase in pH at different time intervals.
Quantification of L-Asparaginase activity from 9 test isolates at different time intervals showed the activity to
be in the range of 0.2173-7.5642 u/ml. Strains of A. niger were found to be the strongest producers, followed
by A. terreus. Conclusions: The present study reveals 70% of the marine-derived fungal isolates tested produced
L-Asparaginase. While there is a need to search for better sources of L-Asparaginase, marine-derived
fungi could be promising candidates.
Keywords: Anticancer potential, Aspergillus, Cladosporium, gulf of khambhat, L-asparaginase, marinederived
fungi, west coast of India.
Rights & PermissionsPrintExport