Acute and subchronic toxicity studies on safety assessment of Paecilomyces tenuipes N45 extracts

Author(s): Linna Du, Yan Liu, Chungang Liu, Qingfan Meng, Jingjing Song, Di Wang, Jiahui Lu, Lirong Teng, Yulin Zhou, Lesheng Teng

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 18 , Issue 8 , 2015

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Abstract:

Paecilomyces tenuipes, one of the commonly used Chinese medicinal fungus, has received much attention over the world, which possesses various active compounds and biological activities. However, little toxicological information is available. Therefore, the present study evaluated the potential toxicity of aqueous and ethanol extracts of Paecilomyces tenuipes N45 via acute and subchronic administration in mouse and rat, respectively. For improving the extraction rate of aqueous extract, response surface methodology (RSM) was employed to optimize the extraction condition first in this paper. The obtained optimal extract conditions were temperature 80 °C, liquid-solid ratio 50 mL·g-1 and time 3 h. In the acute toxicity test, aqueous and ethanol extracts caused neither mortality nor toxicological signs, and the maximum tolerance dose was estimated over 15 g/kg. No mortality or adverse effects was observed in subchronic toxicity studies. No significant difference in bodyweight, relative organ weight or hematological parameters was noted during the experiment. Comparing with nontreated rats, ALT, K and BUN levels were changed in experimental group detecting via biochemical analysis. No abnormality of internal organs was noted between treatment and control groups in gross and histopathological examinations. Our present study suggested that the tolerance dose of the Paecilomyces tenuipes N45 extracts were more than 15 g/kg and no-observed-adverse-effect level (NOAEL) of the extracts for both male and female rats after 90-day adminstation. Additionally, the extracts may possess renal-protective and hepato-protective effects.

Keywords: Biochemistry analysis, hematology analysis, histopathological analysis, Paecilomyces tenuips, response surface methodology, toxicity.

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Article Details

VOLUME: 18
ISSUE: 8
Year: 2015
Page: [809 - 818]
Pages: 10
DOI: 10.2174/1386207318666150803141448
Price: $65

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