Title:Towards Personalized Regenerative Cell Therapy: Mesenchymal Stem Cells Derived from Human Induced Pluripotent Stem Cells
VOLUME: 11 ISSUE: 2
Author(s):Lin Lin, Lars Bolund and Yonglun Luo
Affiliation:Department of Biomedicine, Aarhus University, Wilhelm Meyers Alle 4, DK-8000, Aarhus C, Denmark.
Keywords:BM-MSC, cell therapy, gene therapy, induced pluripotent stem cells (iPSCs), Mesenchymal stem cells (MSC), regenerative
medicine, tissue engineering.
Abstract:Mesenchymal stem cells (MSCs) are adult stem cells with the capacity of self-renewal and
multilineage differentiation, and can be isolated from several adult tissues. However, isolating MSCs
from adult tissues for cell therapy is hampered by the invasive procedure, the rarity of the cells and
their attenuated proliferation capacity when cultivated and expanded in vitro. Human MSCs derived
from induced pluripotent stem cells (iPSC-MSCs) have now evolved as a promising alternative cell
source for MSCs and regenerative medicine. Several groups, including ours, have reported successful
derivation of functional iPSC-MSCs and applied these cells in MSC-based therapeutic testing. Still,
the current experience and understanding of iPSC-MSCs with respect to production methods, safety
and efficacy are primitive. In this review, we highlight the methodological progress in iPSC-MSC research, describing the
importance of choosing the right sources of iPSCs, iPSC reprogramming methods, iPSC culture systems, embryoid body
intermediates, pathway inhibitors, basal medium, serum, growth factors and culture surface coating. We also highlight
some progress in the application of iPSC-MSCs in direct cell therapy, tissue engineering and gene therapy.
