Malaria is the world’s most fatal disease - causing up to 2.7 million deaths annually all over
the world. The ability of organisms to develop resistance against existing antimalarial drugs
exacerbates the problem. There is a clear cut need for more effective, affordable and accessible drugs
that act by novel modes of action. Glutathione synthetase (GS) from Plasmodium falciparum
represents an important potential drug target due to its defensive role; hence ceasing the respective
metabolic step will destroy the parasite. A three dimensional model of Plasmodium GS was
constructed by de novo modelling method and potential GS inhibitors were identified from a library of
glutathione (GSH) analogues retrieved from Ligand-info database and filtered using Lipinski and ADME rules. Two
common feature pharmacophore models were generated from the individual inhibitor clusters to provide insight into the
key pharmacophore features that are crucial for the GS inhibition. Molecular docking of selective compounds into the
predicted GS binding site revealed that the compound CMBMB was the best GS inhibitor when compared to the standard
reference Chloroquine (CQ). This was taken as indicating that CMBMB was the best effective and safest drug against P.
Keywords: ADME, drug targets, Glutathione synthetase, molecular docking, P. falciparum, pharmacophore.
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