Background: Long-term exposure to arsenic has been linked to cancer in
different organs and tissues, including skin. Methods: Here, non-malignant human
keratinocytes (HaCaT) were exposed to arsenic and its effects on microRNAs (miRNAs;
miR) expression were analyzed via miRCURY LNA array analyses. Results: A total of 30 miRNAs were found differentially
expressed in arsenic-treated cells, as compared to untreated controls. Among the up-regulated miRNAs, miR-
21, miR-200a and miR-141, are well known to be involved in carcinogenesis. Additional findings confirmed that those
three miRNAs were indeed upregulated in arsenic-stimulated keratinocytes as demonstrated by quantitative PCR assay.
Furthermore, bioinformatics analysis of both potential cancer-related pathways and targeted genes affected by miR-21,
miR-200a and/or miR-141was performed. Results revealed that miR-21, miR-200a and miR-141 are implicated in skin
carcinogenesis related with melanoma development. Conclusion: Conclusively, our results indicate that arsenic-treated
keratinocytes exhibited alteration in the miRNAs expression profile and that miR-21, miR-200a and miR-141 could be
promising early biomarkers of the epithelial phenotype of cancer cells and they could be potential novel targets for melanoma
Keywords: Arsenite, cancer, carcinogenesis, miR-21, miR-200a, miR-141, miRNA-based cancer therapy, skin cancer.
Rights & PermissionsPrintExport