Objective: Bone is one of the most common sites of metastases in human
small cell lung cancer (SCLC) which is often associated with skeletal-related events and poor prognosis. To explore the
significance of Paeonol (Pae) in SCLC, we hypothesize that Pae can inhibit cell proliferation, migration and invasion,
finally inhibit bone metastases of SCLC.
Methods: The effects of Pae on proliferation of SCLC were detected by MTT assay in vitro. The effects of Pae on
migration and invasion of SCLC were detected by Transwell assay in vitro. Furthermore, bone chip adhesion assay was
used to explore the effects of Pae on the strength of bone adhesion and metastatic ability in SCLC cell line.
Results: MTT results showed that Pae suppressed SCLC cell proliferation in a dose-dependent manner. Transwell assay
results showed Pae suppressed SCLC cell migration and invasion in a dose-dependent manner. Furthermore, the cells
treated by Pae showed decreased ability of bone adhesion and bone metastases in vitro compared with control.
Conclusions: The study indicated Pae could suppress bone metastases of SCLC cells and Pae might be a promising
medicine for therapy of SCLC bone metastases.