Harmine Induces Gastric Cancer Cell Apoptosis through the ROSmediated PI3K/AKT Signaling Pathway

Author(s): Qingqing Ding, Zhuoyun Wang, Ke Ma, Qi Chen

Journal Name: Current Signal Transduction Therapy

Volume 10 , Issue 2 , 2015

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Graphical Abstract:


Harmine (HM), a β-carboline alkaloid extracted from the seeds of Peganum harmala L., has been reported to have antitumor effects. However, the effects and mechanism of action of HM in human gastric cancer cells remain unclear. This study evaluated the antitumor effects of HM in human gastric cancer in vitro. The in vitro cytotoxicity test indicated that HM inhibits gastric cancer cell viability in a dose- and time-dependent manner. Intracellular ROS production was measured using the fluorescent substrate DCFH-DA. The percentage of apoptotic cells was determined by flow cytometry analysis and DAPI staining. Western blot analysis showed the expression of p-AKT, Bcl-2, Bax and caspase-3 in SGC-7901 cells treated with various agents. We demonstrated that HM induces gastric cancer cell apoptosis through the ROS-mediated PI3K/AKT signaling pathway. Therefore, data from this study not only confirm the potential of HM for the treatment of gastric cancer but also offer a promising anticancer therapeutic strategy for treating gastric cancer.

Keywords: Anticancer, apoptosis, gastric cancer, harmine, reactive oxygen species.

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Article Details

Year: 2015
Published on: 14 September, 2015
Page: [112 - 118]
Pages: 7
DOI: 10.2174/1574362410666150625190713
Price: $65

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