Title:The Protective Effects and Mechanism of Resveratrol-loaded Nanoparticles on HK-2 Cells Suffering from Hypoxia-reoxygenayion
VOLUME: 10 ISSUE: 2
Author(s):Hailun Li, Yu Hu, Yong Xu, Lili Zhong, Yongtao Lin, Xi Da, Weinan Yu and Donghui Zheng
Affiliation:Department of Endocrinology, the Affiliated Huai�an Hospital of Xuzhou Medical College, Huai�an, 223002, China.
Keywords:Cell apoptosis, human renal proximal tubular epithelial cells(HK-2 cells), hypoxia reoxygenation, ischemiareperfusion
injury, resveratrol, resveratrol-loaded nanoparticles.
Abstract:Kidney ischemia-reperfusion injury is a serious illnesses which could
threaten to human health. However, there is lack of effective therapy for the disease.
Recently, resveratrol (Res) has been reported as a potential antioxidant in treatment
of ischemia/reperfusion injury through attenuating oxidative stress and apoptosis.
Because of its insolubility and short half-time, the application of Res is limited. Latest evidence reveals the possibility of
developing nanoparticle-based delivery systems with improved solubility, stability and cytotoxicity of lipophilic drug.
Here, we use Res-loaded nanoparticles (Res-NPs) to evaluate the protective effect of Res-NPs on human renal proximal
tubular epithelial (HK-2 cells) suffering hypoxia-reoxygenayion in vitro. The hypothesis is raised that Res-NPs could
demonstrate enhanced renal protection compared to an equivalent dose of free Res at lower concentration. Res-NP can
significantly improve the survival rate of HK-2 cells and reduce the number of apoptosis cell. It also can reduce the
activity of reactive oxygen species (ROS), decrease the level of Caspse-3 & Bax and increase the level of Bcl-2. Res-NP
can inhibit hypoxia reoxygenation of HK-2 cells apoptosis better than Res, which mechanism is related to its antioxidation.
Res-NPs could be a potential treatment needing intensive research for ischemia/reperfusion.
