Title:Serum of Blood Separation by Means of Dynamic Electrochromatography in a Centrifugal and Electromagnetic Gradient
VOLUME: 8 ISSUE: 3
Author(s):Andrzej Jan Dyszkiewicz
Affiliation:LABIOT Laboratory of Biotechnology Cieszyn, Poland.
Keywords:Centrifugal gradient, electric centripetal force, electromagnetic gradient, electrochromatography,
multigradient electrochromatography, quantum-preparative techniques, quick analytic systems, radial
separation phase, relaxation electrical dipole, relaxation spin imbalance, steering buffer parameters, transversal
separation phase.
Abstract:The paper presents the dynamic association of several gradients of separation
with electrophoresis and the laboratory implementation of a prototype device for dynamic
electrochromatography in a centrifugal and electromagnetic gradient (DECGOE), based on
patent PL171643. The object of the study was to compare the separation capacity and repeatability of the location
of edge parameters (xx(1), yy(1), d) of the contours of a proteinogram fraction for conventional electrophoresis
(EF), radial SR-DECGOE separation and three variants of radial-transverse SR-SPM, SR-SPE, SRSPRRM
distribution as well as the reproducibility of the following prototype parameters: PV(1), PV(2), PV(3)[V]
potentials, PV(3)[F] frequency, ω(1) and ω(2) rotational speed, MC mixer chamber buffer [pH] and volume[ml],
and B [T] magnetic induction. Human blood serum was used in the study, 50 samples for each separation
type. The highest rate of fraction formation was registered for the SR-SPRRM (8,07/s) variant. The percentage
values of standard deviations for the edge parameters of proteinograms were the lowest for conventional
electrophoresis (EF), slightly higher for radial distribution SR, while among the radial-transverse separations,
the highest accuracy was obtained for the SR-SPRRM variant.
