Title:Vybrant DyeCycle Violet Stain Discriminates Two Different Subsets of CD34+ Cells
VOLUME: 11 ISSUE: 1
Author(s):Cristian Nunez-Espinosa, María D. Garcia-Godoy, Ines Ferreira, Juan G. Rios-Kristjansson, David Rizo-Roca, Laura G. Rico, Gerard Rubi-Sans, Carlos Palacio, Joan R. Torrella, Teresa Pages, Mike D. Ward, Gines Viscor and Jordi Petriz
Affiliation:Josep Carreras Leukemia Research Institute, Crta. de Can Ruti, Camí de les Escoles s/n. Edifici IMPPC, 08916 Badalona (Barcelona), Spain.
Keywords:CD34, flow cytometry, P-glycoprotein, Stem cells, Vybrant DyeCycle Violet.
Abstract:Introduction: Studies are needed to understand the role of CD34 expressing cells with regard
to efficient engraftment, especially in the adjuvant treatment of cancer. Materials and Methods: In
this study we have used a modified method in our laboratory for routinely counting CD34+ cells. Unlysed
whole blood samples were stained with the DNA-selective and cell membrane-permeant Vibrant
DyeCycle Violet stain. Results: CD34+ cells exhibit a consistent and differential Vybrant Dye Cycle
Violet staining pattern. Based on their different DCV intensity, we classified these subpopulations as
CD34+/DCVhigh and CD34+/DCVlow cells. In general, DCVhigh cells are about 12-times brighter than DCVlow cells.
Conclusion: DCV staining may be used to discriminate subsets of CD34+ cells similarly to other methods which have
previously defined different functional properties that can be related to the characterization, resolution, and purification of
primitive hematopoietic stem cells in combination with specific useful markers for multicolor flow cytometric measurements.
