Hydrophobic alginate derivative was prepared by the modification of alginate with methyl
oleate. The synthesized oleate alginate ester (OAE) conjugate was characterized by FTIR and 1HNMR
analysis. Results of critical aggregation concentration (CAC) revealed that OAE conjugate had low
CAC and was prone to form self-assembled nanoparticles in aqueous medium. Curcumin loaded OAE
nanoparticles (Cur-OAE Nps) were developed by a simple sonication method and the physicochemical
parameters of the nanoparticles such as zeta potential, size distribution and drug encapsulation were
characterized. The results showed that zeta potential of the prepared nanoparticles was -55.4±0.91 mV
and the average size was about 200 nm. A significant enhancement in aqueous solubility and stability of curcumin were
observed after encapsulation into OAE nanoparticles. With the increase of curcumin concentration, loading efficiency increased
but encapsulation efficiency decreased. The in vitro release profile exhibited significant sustained release pattern
with initial burst release followed by a slower release over a period of one week. Cytotoxicity assay against MCF-7cells
showed that Cur-OAE Nps had slow and continuous cytotoxic effect. Furthermore, in vitro cell uptake study revealed that
cell uptake of curcumin from OAE nanoparticles was sustained and both were time and concentration dependent. Therefore,
the developed Cur-OAE Nps might be promising candidates for curcumin delivery to cancer cells.
Keywords: Alginate, Curcumin, Cytotoxicity, Cell uptake studies, Methyl-oleate, Nanoparticles, Self - assembled.
Rights & PermissionsPrintExport