Title:High Cytotoxicity and Apoptotic Effects of Natural Bioactive Benzofuran Derivative on the MCF-7 Breast Cancer Cell Line
VOLUME: 18 ISSUE: 5
Author(s):Afsane Soleimani, Jahanbakhsh Asadi, Faramarz Rostami-Charati and Roghaye Gharaei
Affiliation:Departments of Chemistry, Faculty of Science, Gonbad Kavous University, P.O.Box 163, Gonbad, Iran
Keywords:Apoptosis, benzofuran derivative, Cytotoxicity, MCF-7 breast cancer cell line, petasites hybridus.
Abstract:This study was focused on evaluation of the cytotoxicity and apoptotic
affects of benzofuran derivative on MCF-7 breast cancer cell line. This effective
compound was isolated from the root of Petasites hybridus plant. For experiments, the
MCF-7 cells were treated with several concentrations (0-500μM) of 1-(6-hydroxy-2-
isopropenyl-1-benzofuran-5-yl)-1-ethanone 1 at different times. In this study, test of neutral red was also employed for
cytotoxicity assay and quantity of P53, P21. Bax genes expression was analyzed using Real-Time PCR and ELISA
techniques. Results show that compound 1 has cytotoxicity and apoptotic effects on Human breast cancer (Michigan
Cancer Foundation-7) MCF-7 cells.
