Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Author(s): Rafael C. Rodrigues, Karel Hernandez, Oveimar Barbosa, Nazzoly Rueda, Cristina Garcia-Galan, Jose C. S. dos Santos, Angel Berenguer-Murcia, Roberto Fernandez-Lafuente

Journal Name: Current Organic Chemistry

Volume 19 , Issue 17 , 2015

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact in enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

Keywords: Hydrophobic supports, enzyme destabilization, interfacial activation of lipases, modulation of lipase properties, supported ionic liquids.

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Article Details

Year: 2015
Published on: 05 August, 2015
Page: [1707 - 1718]
Pages: 12
DOI: 10.2174/1385272819666150429231728
Price: $58

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