Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved
in many organs. Previous report indicated that the serum of Korean patients with BD stimulates
membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant
human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF-TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity
against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of
BD in Han Chinese population.
Keywords: Autoantigen, Behcet's disease, HnRNP A2/B1, Protein Overexpression and Purification.
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