Abstract
Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF-TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.
Keywords: Autoantigen, Behcet's disease, HnRNP A2/B1, Protein Overexpression and Purification.
Protein & Peptide Letters
Title:Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients
Volume: 22 Issue: 6
Author(s): Jinghui Liang, Weikang Yang, Xiangyu Meng, Peng Chen and Hongwu Du
Affiliation:
Keywords: Autoantigen, Behcet's disease, HnRNP A2/B1, Protein Overexpression and Purification.
Abstract: Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF-TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.
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Cite this article as:
Liang Jinghui, Yang Weikang, Meng Xiangyu, Chen Peng and Du Hongwu, Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients, Protein & Peptide Letters 2015; 22 (6) . https://dx.doi.org/10.2174/0929866522666150428121442
DOI https://dx.doi.org/10.2174/0929866522666150428121442 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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