Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients

Author(s): Jinghui Liang, Weikang Yang, Xiangyu Meng, Peng Chen, Hongwu Du

Journal Name: Protein & Peptide Letters

Volume 22 , Issue 6 , 2015


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Abstract:

Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF-TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.

Keywords: Autoantigen, Behcet's disease, HnRNP A2/B1, Protein Overexpression and Purification.

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Article Details

VOLUME: 22
ISSUE: 6
Year: 2015
Published on: 25 May, 2015
Page: [504 - 508]
Pages: 5
DOI: 10.2174/0929866522666150428121442
Price: $65

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