This study was conducted to identify and compare natural antioxidant based plant sources. Polyphenols
are the main constituents of the natural antioxidants obtained from fruits, leaves, and seeds etc. In this
work, four types of seeds from Trachyspermum ammi (Ajwain), Brassica alba (Mustard), Trigonella foenum
graecum (Fenugreek) and Papaver somniferum (Poppy) were selected and their extracts were tested for antioxidant
activity (AOA) by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and chelating methods. Total phenolic contents
were found in the range of 1015-6900 mg/100 g (p<0.5). Antioxidant activity by DPPH radical scavenging
and chelating methods was found to be in the range of 44-95% and 33-77% (p<0.5), respectively. This
study revealed that T.ammi is a potent source of natural antioxidant and can be used to replace synthetic antioxidant.
However mustard, fenugreek and poppy have also proved to be the potential sources of phenolics.
Keywords: Antioxidant activity, chelating effect, DPPH, phenolics content, polyphenols, seeds.
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