Title:Production of β -cyclodextrin from pH and Thermo Stable Cyclodextrin Glycosyl Transferase, Obtained from Arthrobacter mysorens and Its Evaluation as a Drug Carrier for Irbesartan
VOLUME: 12 ISSUE: 4
Author(s):Y. Rajesh, K. Narayanan, M. Sreenivasa Reddy, Vijaya K. Bhaskar, G. Gautham Shenoy, V. M. Subrahmanyam and J. Venkata Rao
Affiliation:Dept. of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal -576 104, India
Keywords:β-cyclodextrin, cyclodextrin, irbesartan inclusion complex, thermostable CGTase.
Abstract:Cyclodextrins (CDs) are carrier molecules produced by cyclization of α-1,4-glucans by Cyclodextrin
Glycosyl Transferase (CGTase). These torus shaped molecules have hydrophobic cavity and
hydrophilic shell making them useful in pharmaceutical, food, textile, pesticide and cosmetic industries.
In this study, culture conditions for the production of CGTase by organism belonging to Arthrobacter
genus obtained from a paddy field soil were optimized by single parameter mode. Soluble
starch, yeast extract and magnesium sulphate played an important role in CGTase production. Percentage
increase in CGTase yield under optimized conditions was 396.77%. The enzyme precipitated by
60% ammonium sulphate was purified using DEAE-sepharose. The molecular weight of the purified protein as determined
by SDS-PAGE was 75 kDa. Purified CGTase was thermostable and stable over a wide pH range. Dissolution studies
on β -cyclodextrin-Irbesartan complex revealed that β -CDs formed were useful in preparing immediate release oral
dosage forms.
