Differentiating embryonic stem cells into a specific lineage or cell type is one of the most
investigated areas of stem cell research; however it is wrought with hurdles. The differentiation process
during embryogenesis is greatly influenced by physiochemical factors. They direct key genes for
lineage commitment, which in turn are responsible for patterning into highly organized tissues resulting
in organ formation. In this review, we have focused on the influence of physiochemical factors on
the differentiation process in embryonic stem cells, which mimics, embryogenesis in vivo.