The rapid development of the analytical instrumentation, used in routine analysis tests in
both laboratory and industrial scale, has been accompanied by the application of different smart software.
Such combinations are recommended since they are inexpensive while at the same time they can
readily ensure reliable results in short timeframe. The present work is an attempt to provide simple solutions
in difficult analytical issues (e.g. the simultaneous determination of binary and/or more complex
mixtures) through the development of three different methods. Therefore, metformin, phenformin,
chlorpopamide, glipizide, tolbutamide and glibenclamide were employed to study techniques
which differ in terms of experimental equipment, mathematical approach as well as data processing. The methods applied
are: i) UV Spectrophotometry coupled with the chemometric technique derivative ratio or the multivariate method Partial
Least Squares to Latent Structures (PLS), and ii) High Performance Liquid Chromatography (HPLC) in combination with
experimental design methodology and crossed D-optimal process. Accuracy control includes preparation of a series of
samples (solutions and compact formulations) with different drug concentration whose determination was tested. It was
crucial to verify that the used techniques were versatile and handy as well as to evaluate them in terms of recovery and repeatability
percentages and linearity.