A multi-functional “turn-off” fluorescent probe, N-[[(6-Amino-1,2,3,4-tetrahydro-1,3-
dimethyl-2,4-dioxo-5-pyrimidinyl)amino]thioxomethyl]- benzamide, was synthesized and characterized.
The probe showed high sensitivity and selectivity towards copper(II), iron(III) and silver(I) ions
via quenching of its fluorescence. The detection limits were found to be 4.0 – 16.0 ppb for the assayed
cations. The thiourea linked uracil fluorescent probe is proved to be sensitive, selective, applicable in real samples and
promise for practical applications.