Alternative Biomaterial Substrates for Human Embryonic Stem Cell Culture
Pp. 65-91 (27)
Nicholas R. Forsyth
The additive effect of stem cell therapy and biomaterial substrates provides
exciting opportunities for tissue engineering and regenerative medicine applications.
Nanofibrous substrates can be fabricated to mimic the nano-architectural structure of
specific, native tissue extracellular matrix. This provides topographical structure and
contact guidance, which can impact stem cell biology as well as direct their
differentiation towards specific lineages. This chapter highlights nanofibrous substrates
as an alternative tool for the expansion and differentiation of embryonic stem cells.
Future applications of such technology could promote the use of hESC-derived cells for
Biomimetic, clonogenicity, embryonic stem cells, electrospinning,
nanotechology, feeder layers, MatrigelTM, nanofiber substrates, synthetic