Inositol & its Phosphates: Basic Science to Practical Applications

Inositol and its phosphates are ubiquitous across most life forms, wherein they play important roles in cellular integrity and functions such as signal transduction, DNA damage repair ion transport ...
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Analysis & Assays of Inositol and Inositol Phosphates

Pp. 50-56 (7)

A.K.M. Shamsuddin and Guang-Yu Yang

Abstract

While the quantitation of inositol in biological specimens and that of InsP6 in food is rather simple and the methodologies are well-established, accurate quantitation of inositol phosphates, especially InsP6 in biological specimens in vivo has not been easy, many being dogmatic about even its presence in human tissues. In spite of that, it appears that finally accurate and specific methods to quantitate and follow InsP6 in cells, tissues, plasma, urine etc. may have been developed that might be useful in both research and clinical laboratories.

Keywords:

Fluorescence, HPLC, gas chromatography, GC-MS, ion-exchange chromatography, LC-MS.

Affiliation:

The University of Maryland School of Medicine 10 S. Pine Street, MSTF-700 Baltimore, MD 21201-1116 USA., Northwestern University Feinberg School of Medicine 303 E Chicago Ave, Ward 4-115 Chicago, IL 60611 USA.