In the current study we reported cultivation, extraction procedure, analysis and preliminary characterization of
the aqueous extract from Cereus peruvianus callus culture and evaluated its anti ulcerogenic activity in vivo models of experimental
ulcers in Wistar rats. The obtained aqueous extract from callus (AC) was dialyzed and subjected to freeze-thaw
process, providing a possible polysaccharide. The carbohydrate and protein contents of the aqueous extract were estimated
at 53.4% and 0.66%, respectively, composed primarily of galactose, arabinose and galacturonic acid, with minor amounts
of glucose. This appeared heterogeneous when analyzed by high-performance size-exclusion chromatography and a multiangle
laser light scattering detector (HPSEC-MALLS). The AC was found to be significantly effective against ethanolinduced
lesions but was ineffective against indomethacin-induced lesions. The callus culture of C. peruvianus is an alternative
source for the synthesis of substances originally produced by plants. The calluses grown indefinitely in vitro under
controlled conditions are stable tissues, and the aqueous extract from calluses may be used instead of fully developed
plants using the protocols described in this study.
Keywords: Aqueous extract, antiulcer activity, Cactaceae, callus culture, Cereus peruvianus, plant biotechnology, polysaccharides.
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