Primary Characterization and Evaluation of Anti Ulcerogenic Activity of an Aqueous Extract from Callus Culture of Cereus peruvianus Mill. (Cactaceae)

Author(s): Milena O. Jayme, Franciele Q. Ames, Ciomar A. Bersani-Amado, Maria de Fatima P.S. Machado, Claudete A. Mangolin, Regina A.C. Goncalves, Arildo J.B. de Oliveira

Journal Name: Current Pharmaceutical Biotechnology

Volume 16 , Issue 5 , 2015

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Abstract:

In the current study we reported cultivation, extraction procedure, analysis and preliminary characterization of the aqueous extract from Cereus peruvianus callus culture and evaluated its anti ulcerogenic activity in vivo models of experimental ulcers in Wistar rats. The obtained aqueous extract from callus (AC) was dialyzed and subjected to freeze-thaw process, providing a possible polysaccharide. The carbohydrate and protein contents of the aqueous extract were estimated at 53.4% and 0.66%, respectively, composed primarily of galactose, arabinose and galacturonic acid, with minor amounts of glucose. This appeared heterogeneous when analyzed by high-performance size-exclusion chromatography and a multiangle laser light scattering detector (HPSEC-MALLS). The AC was found to be significantly effective against ethanolinduced lesions but was ineffective against indomethacin-induced lesions. The callus culture of C. peruvianus is an alternative source for the synthesis of substances originally produced by plants. The calluses grown indefinitely in vitro under controlled conditions are stable tissues, and the aqueous extract from calluses may be used instead of fully developed plants using the protocols described in this study.

Keywords: Aqueous extract, antiulcer activity, Cactaceae, callus culture, Cereus peruvianus, plant biotechnology, polysaccharides.

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Article Details

VOLUME: 16
ISSUE: 5
Year: 2015
Page: [462 - 467]
Pages: 6
DOI: 10.2174/1389201016666150303154342
Price: $65

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