Title:Advances in Reprogramming to Pluripotency
VOLUME: 10 ISSUE: 3
Author(s):Suad Alateeq, Patrick R. J. Fortuna and Ernst Wolvetang
Affiliation:Australian Institute for Bioengineering and Nanotechnology, Level 4, Building 75, University of Queensland, St Lucia QLD 4072, Australia.
Keywords:iPSCs, nuclear reprogramming, pluripotent stem cells, reprogramming, reprogramming strategies, reprogramming
technology.
Abstract:Pluripotent stem cells (PSCs) derived from somatic cells represent a powerful experimental
tool for investigating the molecular mechanisms underlying the disease phenotype; with prospects to
advance medical therapies. They also have significant potential as a renewable source of autologous
cells for cellular therapy. Various approaches for PSC derivation from somatic cells have been reported
in the literature. The method used for reprogramming is particularly relevant as it may affect the characteristics
and quality of PSCs. This review will present an overview of the basic strategies and methods
for reprogramming to pluripotency. These strategies will be briefly discussed in the context of how
the mechanism of reprogramming could influence PSC characteristics with respect to safety and quality. Aspects of the reprogramming
approach that can influence PSC properties, such as culture conditions and donor cell source, are also discussed.
