Separation and Determination of Phenylpropanoid Glycosides in Rhodiola by Microemulsion Electrokinetic Chromatography

Author(s): Bin Tang, Dan Guo, Xia Lin, Jin Yan, Hongqin Yang, Hui Li

Journal Name: Current Pharmaceutical Analysis

Volume 11 , Issue 3 , 2015


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Abstract:

A novel microemulsion electrokinetic chromatography (MEEKC) method for the simultaneous separation and quantitative determination of phenylpropanoid glycosides in Rhodiola L. was developed. The effects of many parameters influencing separation characteristics were optimized. Retention factor met rosarin<rosavin<rosin at the same 2-propanol concentration. Both selectivity and resolution of rosarin and rosavin increased with increasing 2-propanol content. The similar chemical structure led to a small difference in theoretical plates. The resolution of rosarin and rosavin showed greater improvements than rosavin and rosin with increasing buffer pH. According to the separation characteristics, the optimal running buffer was determined: 80 mmol/L sodium dodecyl sulfate, 1.0% n-heptane, 7.5% 1-buatnol, 22.5% 2-propanol, and 10 mmol/L sodium borate buffer at pH 9.25. Validation showed that this simple MEEKC method possesses feasible accuracy and precision. Furthermore, the developed method successfully quantified three phenylpropanoid glucosides in the Rhodiola rosea sample extracts and three oral liquids.

Keywords: Capillary electrophoresis, microemulsion electrokinetic chromatography, phenylpropanoid glycosides, rhodiola, rosarin, rosavin, rosin.

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Article Details

VOLUME: 11
ISSUE: 3
Year: 2015
Published on: 05 May, 2015
Page: [224 - 230]
Pages: 7
DOI: 10.2174/1573412911666150121231557
Price: $65

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