A novel microemulsion electrokinetic chromatography (MEEKC) method for the simultaneous
separation and quantitative determination of phenylpropanoid glycosides in Rhodiola L. was
developed. The effects of many parameters influencing separation characteristics were optimized. Retention
factor met rosarin<rosavin<rosin at the same 2-propanol concentration. Both selectivity and
resolution of rosarin and rosavin increased with increasing 2-propanol content. The similar chemical
structure led to a small difference in theoretical plates. The resolution of rosarin and rosavin
showed greater improvements than rosavin and rosin with increasing buffer pH. According to the
separation characteristics, the optimal running buffer was determined: 80 mmol/L sodium dodecyl sulfate,
1.0% n-heptane, 7.5% 1-buatnol, 22.5% 2-propanol, and 10 mmol/L sodium borate buffer at pH 9.25. Validation showed
that this simple MEEKC method possesses feasible accuracy and precision. Furthermore, the developed method successfully
quantified three phenylpropanoid glucosides in the Rhodiola rosea sample extracts and three oral liquids.
Keywords: Capillary electrophoresis, microemulsion electrokinetic chromatography, phenylpropanoid glycosides, rhodiola,
rosarin, rosavin, rosin.
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