Analytical Characterization of Label-Free Immunosensor Subsystems Based on Multi-Walled Carbon Nanotube Array-Modified Gold Interface

Author(s): Masoumeh Zahmatkeshan, Hoda Ilkhani, Malihe Paknejad, Moein Adel, Saeed Sarkar, Reza Saber

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 18 , Issue 1 , 2015


Become EABM
Become Reviewer
Call for Editor

Abstract:

In the present work, we report characterization studies of antigen(Ag)-antibody (Ab) interaction based on the multi-walled carbon nanotube array-modified gold electrode for label-free electrochemical immunosensor. -COOH functionalization of MWCNT was suggested by FTIR spectroscopy. Images from atomic force microscopy (AFM) and RAMAN spectroscopy confirmed the interaction of Ab on the gold interface based on the MWCNT array. A cyclic voltammetric study exhibited a linear response in phosphate buffered saline (PBS) solution including 1mM K3Fe (CN) 6 at pH 7.4 with PSA concentrations over a range of 2 to 40 ngmL-1, and a calculated detection limit of 0.56 ngmL-1.

Keywords: Atomic force microscopy, carbon nanotube, cyclic voltammetry, immunosensor, label free, RAMAN spectroscopy.

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 18
ISSUE: 1
Year: 2015
Published on: 12 December, 2014
Page: [83 - 88]
Pages: 6
DOI: 10.2174/1386207318666141212165513

Article Metrics

PDF: 37
HTML: 3