Antiproliferative and Antioxidant Potential of Leaf and Leaf Derived Callus Extracts of Aerva lanata (L.) Juss. Ex Schult. Against Human Breast Cancer (MCF-7) Cell Lines

Author(s): Deepak Kamalanathan, Devarajan Natarajan

Journal Name: The Natural Products Journal

Volume 4 , Issue 4 , 2014

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Graphical Abstract:


Aerva lanata is a prostrate under-shrub used as traditional and modern system of medicine. The Prime of current study was to compare the antiproliferative and antioxidant potential of in vitro and in vivo leaf and callus extracts of Aerva lanata against human breast cancer (MCF 7) cell lines. Anticancer proficiency of methanol extracts of in vitro and in vivo leaf and matured callus extracts were evaluated by 3 - (4, 5 – dimethylthiazole – 2 – yl) - 2, 5 -diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays. Earlier the total antioxidant potential of the different solvent extracts was tested by 2, 2' – azino – bis (3-ethylbenzthiazoline – 6 – sulphonic acid (ABTS) assay. A dose dependent inhibition of cancer cell proliferation was identified in MTT assay. Further, DNA fragmentation assay was performed and the results show the best IC50 values produced best possible digestion of DNA (by apoptosis). The results of radical scavenging activity of ABTS cation assay was recorded promising inhibitory effects of callus, in vivo and in vitro leaf methanol extracts. The inhibition rate was mainly dependent on concentration of extracts. Total phenols, tannins and flavonoids contents of the test samples were quantified. The overall results from the study highlights the use of least concentrations of methanolic callus extracts of plant has potential use in treatment of cancer related diseases in future.

Keywords: ABT’S, Aerva lanata, Callus, DNA fragmentation, MCF – 7, MTT.

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Article Details

Year: 2014
Page: [271 - 279]
Pages: 9
DOI: 10.2174/2210315504666141119223215
Price: $25

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PDF: 27