Achyrobichalcone is a biflavonoid recently found in Achyrocline satureioides. This substance
has unprecedented chemical structure and occurrence, but resembles other bioactive bichalcones, which
have important pharmacological properties, such as anticancer activity. The major challenge for evaluation of the
physicochemical and biological properties of this new molecule is the isolation step, which affects the purity and yield of
the isolated product. Thus, the objective of this work was to develop a semi-preparative method for achyrobichalcone isolation
from Achyrocline satureioides by high-speed countercurrent chromatography. The high-speed countercurrent chromatography
separation was achieved in two steps. In the first step, an enriched fraction of achyrobichalcone from the
freeze-dried extract was obtained, using the solvent system hexane-ethyl acetate-methanol-water 0.8:1:0.8:1, v/v. The purification
of achyrobichalcone from the enriched fractions was achieved by further high-speed countercurrent chromatography
fractionation with hexane-ethyl acetate-methanol-water 0.9:0.9:0.8:1, v/v. The final isolated product was obtained
using preparative thin layer chromatography and crystallization procedure. A yellow semi-crystalline solid with purity
close to 90% was obtained as the final product. The mass recovery of achyrobichalcone isolation was near 67%. The
structural identification from spectroscopic and chromatographic techniques confirmed the achyrobichalcone structure.
This is the first report of achyrobichalcone isolation on a semi-preparative scale by high-speed countercurrent chromatography.
This method afforded achyrobichalcone in good yield and purity for further biopharmaceutical studies.
Keywords: Achyrobichalcone, Achyrocline satureioides, biflavonoid, flavonoid, high-speed countercurrent chromatography
(HSCCC), isolation, semi-preparative isolation.
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