Current delivery platforms are typically designed for prolonged circulation that favors superior
accumulation of the payload in the targeted tissue. The design of efficient surface modifications
determines both a longer circulation time and targeting abilities of particles. The optimization of synthesis
protocols to efficiently combine targeting molecules and elements that allow for an increased
circulation time can be challenging and almost impossible when several functional elements are needed. On the other
hand, in the last decade, the development of bioinspired technologies was proposed as a new approach with which to increase
particle safety, biocompatibility and targeting, while maintaining the synthesis protocols simple and reproducible.
Recently, we developed a new drug delivery system inspired by the biology of immune cells called leukolike vector
(LLV) and formed by a nanoporous silicon core and a shell derived from the leucocyte cell membrane. The goal of this
study is to investigate the protein content of the LLV. Here we report the proteomic profiling of the LLV and demonstrate
that our approach can be used to modify the surface of synthetic particles with more than 150 leukocyte membraneassociated
proteins that determine particle safety, circulation time and targeting abilities towards inflamed endothelium.
Keywords: Bio-mimetic camouflage, drug delivery, leukocyte, leukolike vector, membrane, nanotechnology, nanoparticles,
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