Title:Rapid Screening of Different Types of Antitumor Compound Groups from Traditional Chinese Medicine by Hollow Fiber Cell Fishing with High Performance Liquid Chromatography
VOLUME: 17 ISSUE: 10
Author(s):Li-Sha Zhang, Xuan Chen, Shuang Hu, Xia Sheng and Xiao-Hong Bai
Affiliation:School of Pharmacy, Shanxi Medical School, Taiyuan 030001, P.R. China.
Keywords:Active compound group, cell fishing factor, cells seeding, hollow fiber cell fishing, screen, traditional Chinese
medicine.
Abstract:A novel hollow fiber cell fishing with high performance liquid chromatography (HFCF-HPLC) was extended
and used to screen flavonoid and anthraquinone active compound groups simultaneously from traditional Chinese
medicines (TCMs). In this study, three cells (MCF-7, SGC7901, and MADB-106) were seeded on the inner wall of the
hollow fiber employed to screen bioactive components from TCM water decoction. The variables influencing HFCFHPLC,
such as cell seeding time, screening stirring rate and time, and active compound concentration, were investigated
and optimized. The surface property of the hollow fiber seeded with cells, the cell survival rate under different conditions,
the nonspecific binding between active centers in the fiber and the target compounds, and the repeatability and recovery of
HFCF-HPLC were analyzed and validated. Certain structures of the compounds fished by HFCF-HPLC were identified
after comparing the retention times of the reference substances. To verify preliminarily the binding site between the
bioactive components and cells, we separated the cell membrane and cell organelle from live MCF-7 cells. We then
employed the cell membrane, cell organelle, and the whole cells to screen simultaneously the active compounds. The cell
fishing factor of the active compound was calculated and discussed as the index of cell-drug binding ability in HFCFHPLC.
Tamoxifen as a positive control and indomethacin as a negative control were screened by HFCF-HPLC to verify
the method. The results indicate that HFCF-HPLC is an effective and reliable method for the screening and analysis of
bioactive components. Moreover, this method can be applied to predict bioactive candidates in TCMs.
