The present investigation describes two simple, sensitive and validated spectrophotometric
methods for the determination of Cyclophosphamide (CP) in pure and formulated forms. The methods
are mainly based on the formation of charge transfer (CT) complex, with the reagents 2,3-dichloro-5,6-
dicyano-1,4-benzoquinone (DDQ) (Method A) and p-Chloranilic acid (PCA) (Method B), hence a stable,
1:1 stoichiometric complex was obtained, which shows orangeyellow and pink colors, respectively.
The produced chromogens optical densities were measured at 352 and 522 nm, respectively. The Beer’s
law was tested in the range of 2-16 and 5-50 μg mL-1. The optical characteristics such as molar absorptivity (ɛ), and Sandell’s
sensitivity values were calculated. Other analytical parameters like, Limit of Detection (LOD), Limit of Quantification
(LOQ), accuracy and precisions were evaluated. The proposed methods were successfully applied for the determination
of the CP in tablet formulation with higher accuracy, better recoveries and less than 2% relative standard deviation.