G-protein coupled receptors (GPCRs) are proteins of the plasma membrane, which are characterized by seven
membrane-spanning segments (TMs). GPCRs play an important role in almost all of our physiological and pathophysiological
conditions by interacting with a large variety of ligands and stimulating different G-proteins and signaling cascades.
By playing a key role in the function of our body and being involved in the pathophysiology of many disorders,
GPCRs are very important therapeutic targets. Determination of the structure and function of GPCRs could advance the
design of novel receptor-specific drugs against various diseases. A powerful method to obtain structural and functional information
for GPCRs is the cysteine substituted accessibility method (SCAM). SCAM is used to systematically map the
TM residues of GPCRs and determine their functional role. SCAM can also be used to determine differences in the structures
of the TMs in different functional states of GPCRs.
Keywords: Binding-site crevice, cysteine substituted accessibility method, function, G-protein coupled receptors, methanothiosulfonate
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