A New Validated HPLC-DAD Method for Estimation of Solasodine from Solanum nigrum Linn. Extracts and Formulations

Author(s): Sonal Desai, Pratima Tatke, Satish Gabhe

Journal Name: The Natural Products Journal

Volume 4 , Issue 3 , 2014

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Graphical Abstract:


A new, simple, accurate and precise HPLC-DAD method has been developed and validated for quantitative determination of solasodine in aerial parts of Solanum nigrum Linn. The column used was C18 (150 mm X 4.6 mm I.D., 5 μm) and mobile phase used was acetonitrile: 10 mM ammonium acetate buffer pH 7.7(65:35,v/v).The flow rate of mobile phase and detection wavelength were kept at 2.0 ml/ min and at 205 nm, respectively. The column oven temperature was set at 40 °C. The linearity of the method was found over a range of 300-1500 µg/ml with LOD and LOQ of 100 µg/ml and 300 µg/ml, respectively. The method has shown recoveries of solasodine in the range from 100.12 % -106.67 %, with low intra and inter-day % R.S.D. (0.89% and 1.25%, respectively). The method was successfully applied for quantitative evaluation of three extracts and different Ayurvedic formulations for the content of solasodine.

Keywords: Makoya, solanaceae, Solanum nigrum, Solanum species, solasodine, steroidal alkaloids.

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Article Details

Year: 2014
Published on: 10 December, 2014
Page: [196 - 200]
Pages: 5
DOI: 10.2174/2210315504666141017002102
Price: $25

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